Composite

Part:BBa_K3799003:Design

Designed by: Shubhamay Das,Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-09-30)


Dnase I with promoter(R0010) , RBS(B0034), Terminator(B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists of DNaseI coding sequence(BBa_K3799002) under pLac promoter(BBa_j04500) of Ecoli along with strong RBS(BBa_B0034) and double terminator(BBa_B0015).The coding sequence are optimized for producing DNaseI is Ecoli BL21 host.


Source

This part is obtain synthetically from Twistbioscience and was amplified using Biobrick prefix and suffix primers.

The sequence was codon optimized for Ecoli host.

References

https://www.ncbi.nlm.nih.gov/gene/282217